@article {18709,
	title = {A two-component pre-seeded dermal-epidermal scaffold},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	month = {2014-09-01 00:00:00},
	pages = {4928-4938},
	publisher = {Elsevier},
	abstract = {

We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.

}, keywords = {Amine{\textendash}aldehyde bonding, Bilayered, Hyaluronic acid, Skin defects}, issn = {1742-7061}, doi = {10.1016/j.actbio.2014.08.029}, url = {http://www.sciencedirect.com/science/article/pii/S1742706114003742}, author = {Monteiro, I. P. and Gabriel, D. and Timko, B. P. and Hashimoto, M. and Karajanagi, S. and Tong, R. and Marques, A. P. and Reis, R. L. and Kohane, D. S.} }

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