@article {19004,
	title = {A semiautomated microfluidic platform for real-time investigation of nanoparticles{\textquoteright} cellular uptake and cancer cells{\textquoteright} tracking.},
	journal = {Nanomedicine},
	year = {2017},
	month = {2017-02-10 00:00:00},
	abstract = {

Aims: develop a platform composed of labeled dendrimer nanoparticles and a microfluidic device for real-time monitoring of cancer cells fate. Materials and Methods: The physicochemical and biological characterization of the developed Carboxymethyl-chitosan/poly(amidoamine) (CMCht/PAMAM) dendrimer nanoparticles were performed using TEM, AFM, Zeta Sizer, DSC and cytotoxicity screening. Cancer cell lines derived from different tumor types, including HeLa (Cervical Carcinoma), HCT-116 (Colon Carcinoma) and U87MG (Glioblastoma), were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 3 days (MTS/DNA). Results: Nanoparticles were successfully modified with an average size of 50 nm. Internalization levels go from 87\% to 100\% in static and from 95\% to 100\% in dynamic conditions. Viability levels range from 95\% to 100\% in static and from 90\% to 100\% in dynamic conditions, being HCT the most sensitive to the presence of the NP. Conclusions: the results show different responses to the presence of 0.5 mg.mL-1 dendrimer nanoparticles when comparing static to dynamic conditions, with a tendency towards higher sensitivity when subjected to confinement. This work demonstrated that the proposed microfluidic-based platform allows real-time cell monitoring, which, upon more studies, namely the assessment of the drug release effect, could be used for cancer theranostics.

}, keywords = {cell tracking, circulating tumor cells, Microfluidics, Nanoparticles, theranostics}, issn = {1743-5889}, doi = {10.2217/nnm-2016-0344}, url = {https://www.ncbi.nlm.nih.gov/pubmed/28186438}, author = {Carvalho, M. and Maia, F. R. and Silva-Correia, J. and Costa, B. M. and Reis, R. L. and Oliveira, J. M.} }

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