@article {19123,
	title = {Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns},
	journal = {Advanced Functional Materials},
	volume = {-},
	year = {2017},
	month = {2017-05-24 00:00:00},
	pages = {-},
	publisher = {Wiley Periodicals, Inc.},
	edition = {-},
	abstract = {

Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments.

}, keywords = {Bioactive glass Nanoparticles, nanocomposites, Superhydrophobic-Wettable Micropatterns}, issn = {1616-3028}, doi = {10.1002/adfm.201701219}, url = {http://onlinelibrary.wiley.com/doi/10.1002/adfm.201701219/abstract}, author = {Leite, A. J. and Oliveira, M.B. and Caridade, S. G. and Mano, J. F.} }

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