Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Chitosan/Chondroitin Sulfate Membranes Produced by Polyelectrolyte Complexation for Cartilage Engineering

Abstract

Membranes made of chitosan (CHT) and chondroitin sulfate (CS) are herein presented using a polyelectrolyte complexation sedimentation/evaporation method. The membranes present high roughness and heterogeneous morphology induced by salt crystals. Exposing the membranes to different salt concentrations induces saloplastic behavior, as shown by an increasing water absorption and decreasing stiffness while exposed to increasing concentrations of salt. Establishing contact between two parts of a cut membrane leads to their self-adhesion and maintenance of their stretching ability. The membranes sustain the adhesion of ATDC5 prechondrocyte cells, inducing their rearrangement in cellular aggregates typical of chondrogenesis, and the expression of cartilage markers. Impregnated TGF-β3 remains loaded after 14 days of incubation, releasing only 1.2% of its total loaded mass. CHT/CS polyelectrolyte membranes are here shown as suitable candidates for the biomedical field, namely, for cartilage regeneration.

Journal
Biomacromolecules
Volume
17
Issue
6
Pagination
2178–2188
Publisher
American Chemical Society
ISSN
1526-4602
URL
http://pubs.acs.org/doi/abs/10.1021/acs.biomac.6b00399
Keywords
cartilage, Polyelectrolyte Complexes, saloplastics
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Project
Matryoshkas
Year of Publication
2016
DOI
10.1021/acs.biomac.6b00399
Date Published
2016-05-18
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.