Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Controlling Cancer Cell Fate Using Localized Biocatalytic Self-Assembly of an Aromatic Carbohydrate Amphiphile

Abstract

We report on a simple carbohydrate amphiphile able to self-assemble into nanofibers upon enzymatic dephosphorylation. The self-assembly can be triggered by alkaline phosphatase (ALP) in solution or in situ by the ALP produced by osteosarcoma cell line, SaOs2. In the latter case, assembly and localized gelation occurs mainly on the cell surface. The gelation of the pericellular environment induces a reduction of the SaOs2 metabolic activity at an initial stage (≤ 7 h) that results in cell death at longer exposure periods (≥ 24 h). We show that this effect depends on the phosphatase concentration, and thus, it is cell-selective with prechondrocytes ATDC5 (that express ∼ 15−20 times lower ALP activity compared to SaOs2) not being affected at concentrations ≤ 1 mM. These results demonstrate that simple carbohydrate derivatives can be used in an antiosteosarcoma strategy with limited impact on the surrounding healthy cells/ tissues.

Journal
Journal of the American Chemical Society
Volume
137
Issue
2
Pagination
576-579
Publisher
American Chemical Society
URL
http://pubs.acs.org/doi/abs/10.1021/ja5111893
Keywords
amphiphile, carbohydrate, self-assembling
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Project
POLARIS
Year of Publication
2015
DOI
10.1021/ja5111893
Date Published
2015-01-21
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.