Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Effect of anatomical origin and cell passage number on the stemness and osteogenic differentiation potential of canine adipose-derived stem cells

Abstract

Mesenchymal stem cells have a great potential for application in cell based therapies, such as tissue engineering. Adipose derived stem cells have shown the capacity to differentiate into several lineages, and have been isolated in many animal species.  Dog is a very relevant animal model to study several human diseases and simultaneously an important subject in veterinary medicine. Thus, in this study we assessed the potential of canine adipose tissue derived stem cells (cASCs) to differentiate into the osteogenic and chondrogenic lineages by performing specific histological stainings, and studied the cell passaging effect on the cASCs stemness and osteogenic potential. We also evaluated the effect of the anatomical origin of the adipose tissue, namely from abdominal subcutaneous layer and from greater omentum. The stemness and osteogenic differentiation was followed by real time RT-PCR analysis of typical markers of mesenchymal stem cells (MSCs) and osteoblasts.

The results obtained revealed that cASCs exhibit a progressively decreased expression of the MSCs markers along passages and also a decreased osteogenic differentiation potential. In the author’s knowledge, this work presents the first data about the MSCs markers profile and osteogenic potential of cASCs along cellular expansion. Moreover, the obtained data showed that the anatomical origin of the adipose tissue has an evident effect in the differentiation potential of the ASCs. Due to the observed resemblances with the human ASCs, we conclude that canine ASCs can be used as a model cells in tissue engineering research envisioning human applications.

Journal
Stem Cell Reviews and Reports
Volume
8
Issue
4
Pagination
1211-1222
Publisher
Springer
ISSN
1550-8943
URL
http://link.springer.com/article/10.1007%2Fs12015-012-9397-0
Keywords
adipose-derived stem cells, Canine model, cell based therapies, osteogenic potential, stemness, Tissue engineering
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Year of Publication
2012
DOI
10.1007/s12015-012-9397-0
Date Published
2012-07-09
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