Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Microfluidic Fabrication of Self-Assembled Peptide-Polysaccharide Microcapsules as 3D Environments for Cell Culture

Abstract

 We report a mild cell encapsulation method based on self-assembly and microfluidics technology. Xanthan gum, an anionic polysaccharide, was used to trigger the self-assembly of a positively charged multidomain peptide. The self-assembly resulted in the formation of a nanofibrous matrix and using a microfluidic device, microcapsules with homogeneous size were fabricated. The properties and performance of xanthan-peptide microcapsules were optimized by changing peptide/polysaccharide ratio and their effects on the microcapsule permeability and mechanical stability were analyzed. The effect of microcapsule formulation on viability and proliferation of encapsulated chondrocytic (ATDCS) cells was also investigated. The encapsulated cells were metabolically active, showing an increased viability and proliferation over 21 days of in vitro culture, demonstrating the long-term stability of the self-assembled microcapsules and their ability to support and enhance the survival of encapsulated cells over a prolonged time. Self-assembling materials combined with microfluidics demonstrated to be an innovative approach in the fabrication of cytocompatible matrix for cell microencapsulation and delivery.

Journal
BIOMACROMOLECULES
Volume
13
Issue
12
Pagination
4039-4048
Publisher
AMER CHEMICAL SOC
ISSN
1525-7797
Keywords
Biomaterials, DESIGN
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Year of Publication
2012
DOI
10.1021/bm301332z
Date Published
2012-12-28
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