Chitosan-intercalated Montmorillonite/Poly (Vinyl alcohol) Nanofibers as a Platform to Guide Neuron-like Differentiation of Human Dental Pulp Stem Cells

last updated: 2017-07-17
ProjectFoReCaST :: publications list
TitleChitosan-intercalated Montmorillonite/Poly (Vinyl alcohol) Nanofibers as a Platform to Guide Neuron-like Differentiation of Human Dental Pulp Stem Cells
Publication TypePapers in Scientific Journals
Year of Publication2017
AuthorsHamidabadi H. G., Rezvani Z., Nazm Bojnordi M., Shirinzadeh H., Seifalian A. M., Joghataei M. T., Razaghpour M., Alibakhshi A., Yazdanpanah A., Salimi M., Mozafari M., Urbanska A. M., Reis R. L., Kundu S. C., and Gholipourmalekabadi M.
Abstract

In this study, we present a novel chitosan- intercalated montmorillonite/poly(vinyl alcohol) (OMMT/PVA)nanofibrous mesh as a microenvironment for guiding differentiation of human dental pulp stem cells (hDPSCs) toward neuronlike cells. The OMMT was prepared through ion exchange reaction between the montmorillonite (MMT) and chitosan. The PVA solutions containing various concentrations of OMMT were electrospun to form 3D OMMT-PVA nanofibrous meshes. The biomechanical and biological characteristics of the nanofibrous meshes were evaluated by ATR-FTIR, XRD, SEM, MTT, and LDH specific activity, contact angle, and DAPI staining. They were carried out for mechanical properties, overall viability, and toxicity of the cells. The hDPSCs were seeded on the prepared scaffolds and induced with neuronal specific differentiation media at two differentiation stages (2 days at preinduction stage and 6 days at induction stage). The neural differentiation of the cells cultured on the meshes was evaluated by determining the expression of Oct-4, Nestin, NF-M, NF-H, MAP2, and βIII-tubulin in the cells after preinduction, at induction stages by real-time PCR (RT-PCR) and immunostaining. All the synthesized nanofibers exhibited a homogeneous morphology with a favorable mechanical behavior.

JournalACS Applied Materials & Interfaces
Volume9
Issue13
Pagination11392–11404
Date Published2017-01-24
PublisherACS
ISSN1944-8252
DOI10.1021/acsami.6b14283
KeywordsChitosan, human dental pulp stem cells, nanofibrous scaffolds
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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