Evaluation of bone turnover markers and serum minerals variations for predicting fracture healing versus non-union processes in adult sheep as a model for orthopedic research

last updated: 2017-07-25
ProjectRECOGNIZE :: publications list
TitleEvaluation of bone turnover markers and serum minerals variations for predicting fracture healing versus non-union processes in adult sheep as a model for orthopedic research
Publication TypePapers in Scientific Journals
Year of Publication2017
AuthorsSousa C. P., Lopez-Peña M., Gúzon F. M., De Abreuc H. V., Luís M. R., Viegas C. A., Camassa J., De Azevedo J. T., Cabrita A. S., Reis R. L., Gomes M. E., and Dias I. R.
Abstract

Bone turnover markers (BTMs) have been considered as an auxiliary method of following the fracture
healing process and for early prediction of impaired bone healing. A better understanding of the potential
of BTMs in this application could allow for earlier interventions and improved patient care. The aim of
this study with a large animal experimental model was to assess the variation of bone formation markers

namely the total alkaline phosphatase (ALP) and its bone-specific isoform (BALP), serum concentration
of intact osteocalcin (OC), N-terminal propeptide type III procollagen (PIIINP) and of bone resorption
markers – namely tartrate resistant acid phosphatase (TRAP) and deoxypyridinoline crosslink (DPD)
during the
first stages of a normal fracture healing process and of a segmental critical size defect (CSD),
which progresses to a non-union process. Thirty healthy female sheep (Portuguese Churra-da-Terra-
Quente breed), approximately 4-years-old, were enrolled in this study. Jugular venous blood samples
were collected pre-operatively and at 1, 2, 3, 4, 6, 8, 10 and 12 post-operative weeks. The animals of the
CSD group showed significant lower serum levels of BALP, OC and significant higher serum PIIINP levels at
early stages of the fracture healing process, compared with animals that progressed in a normal fracture
healing process. Serum BALP, OC and PIIINP levels could be useful as non-invasive auxiliary tools with
other complementary methods for predicting the outcome of traumatic bone fractures.

JournalInjury-international Journal Of The Care Of The Injured
Date Published2017-05-17
PublisherElsevier
ISSN0020-1383
DOI10.1016/j.injury.2017.05.025
URLhttp://www.sciencedirect.com/science/article/pii/S0020138317303509
Keywordsbone turnover markers, critical size defect, Delayed union, Fracture healing process, Non-union, sheep
RightsopenAccess
Peer reviewedyes
Statuspublished

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