|Project||Matryoshkas :: publications list|
|Title||Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate|
|Publication Type||Papers in Scientific Journals|
|Year of Publication||2017|
|Authors||Silva J. M., Garcia J., Reis R. L., Garcia A. J., and Mano J. F.|
Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multilayers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical and chemical properties of these films, enabling the transition from cell-adhesive to cellresistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films.
|Keywords||cell adhesion, cross-linking, fibronectin, surface chemistry, Tissue engineering|
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