@article {18098,
	title = {Hyaluronic acid/poly-L-lysine bilayered silica nanoparticles enhance the osteogenic differentiation of human mesenchymal stem cells},
	journal = { Journal of Materials Chemistry B},
	volume = {2},
	year = {2014},
	month = {2014-08-15 00:00:00},
	pages = {6939-6946},
	publisher = {Royal Society of Chemistry},
	abstract = {
Herein, we evaluate the influence of a PLL{\textendash}HA bilayer on the surface of silica nanoparticles on their capacity to induce the osteogenic differentiation of human bone marrow stem cells (hBMSCs), as a function of their concentration (50 μg mL-1, 25 μg mL-1 and 12.5 μg mL-1). To this purpose, we synthesized silica nanoparticles (diameter of 250 nm; ζ-potential of -25 mV) that were coated with PLL{\textendash}HA (diameter of 560 nm; ζ-potential of -35 mV). The cell viability, cell proliferation, protein quantification (i.e. MTS, DNA and ALP, respectively) and gene expression (of osteogenesis-related genes: ALP, osteocalcin, collagen type I, bone sialoprotein, Runx-2, osteopontin and osterix) were monitored, for 21 days. We observed the overexpression of most of the tested osteogenic transcripts in the hBMSCs cultured with SiO2{\textendash}PLL{\textendash}HA, at concentrations of 25 μg mL-1 and 12.5 μg mL-1. These results indicate that the proposed nanoparticles temporarily improve the osteogenic differentiation of hBMSCs at low nanoparticle concentrations.
},
	keywords = {Hyaluronic acid, layer-by-layer, osteogenesis},
	issn = {2050-750X},
	doi = {10.1039/C4TB01071J },
	url = {http://pubs.rsc.org/en/content/articlepdf/2014/tb/c4tb01071j},
	author = {Amorim, S. and Martins, A. and Neves, N. M. and Reis, R. L. and Pires, R. A.}
}