@article {19010,
	title = {Tuning cell adhesive properties via layer-by-layer assembly of chitosan and alginate},
	journal = {Acta Biomaterialia},
	volume = {n/d},
	year = {2017},
	month = {2017-01-23 00:00:00},
	publisher = {Science direct},
	abstract = {
Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multilayers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical and chemical properties of these films, enabling the transition from cell-adhesive to cellresistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films.
},
	keywords = {cell adhesion, cross-linking, fibronectin, surface chemistry, Tissue engineering},
	issn = {0.1016/j.actbio.2017.01.058},
	doi = {10.1016/j.actbio.2017.01.058},
	url = {http://www.sciencedirect.com/science/article/pii/S174270611730065X},
	author = {Silva, J. M. and Garcia, J. and Reis, R. L. and Garcia, A. J. and Mano, J. F.}
}