@article {19674,
	title = {Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells},
	journal = {Nanomedicine},
	year = {2018},
	month = {2018-09-07 00:00:00},
	abstract = {
Aim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials \& methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115{\textendash}140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35{\textendash}42\%. Drug-release studies demonstrated that around 84\% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25\%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.
},
	keywords = {breast cancer cells, Chitosan, Drug Delivery System, endothelial cells, Fucoidan, gemcitabine, Nanoparticles, Polyelectrolyte complexation},
	issn = {1743-5889},
	doi = {10.2217/nnm-2018-0004},
	author = {Oliveira, C. and Neves, N. M. and Reis, R. L. and Silva, T. H. and Martins, A.}
}