Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Effect of cell density on mesenchymal stem cells aggregation in RGD-alginate 3D matrices under osteoinductive conditions

Abstract

Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered “normal” activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell–cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering.

Journal
Macromolecular Bioscience
Volume
14
Issue
6
Pagination
659-771
URL
http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300567/abstract;jsessionid=902F43BD5677D920D14697F6503D3D0D.f03t02
Keywords
alginate hydrogels, encapsulation, self-assembly, Tissue engineering
Rights
Open Access
Peer Reviewed
Yes
Status
published
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