Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

An efficient and user-friendly method for cytohistological analysis of organoids

Abstract

Organoid culture is a recently developed in-vitro three-dimensional (3D) cell culture technology. It has wide applications in tissue engineering studies. However, histological analysis of organoid is quite complex and tedious for researchers. This study proposes a user-friendly, affordable and efficient method for making formalin-fixed paraffin embedded (FFPE) organoid blocks and Optimal Cutting Temperature compound (OCT) embedded frozen organoid blocks. This method applies a key pre-embedding step for preparing paraffin embedded organoid blocks. This method could be used to process even a small number of organoids with high efficiency. In addition, with minor modifications, the method is readily applied for OCT embedded organoid blocks. The slides generated were ready for H&E staining, immunohistochemistry staining and immunofluorescent staining. The method described in this study can be easily used for routine histological analysis of organoid, and could be performed in general pathology labs and requires no dedicated equipment and reagent.

Journal
Journal of Tissue Engineering and Regenerative Medicine
Publisher
Wiley
ISSN
1932-7005
URL
https://onlinelibrary.wiley.com/doi/10.1002/term.3248
Keywords
culture, method, Organoids
Rights
Closed Access
Peer Reviewed
Yes
Status
published
Project
B-Liver
Year of Publication
2021
DOI
10.1002/term.3248
Date Published
2021-09-17
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