Influence of PDLA nanoparticles size on drug release and interaction with cells

last updated: 2019-01-08
ProjectRL2 – SCN :: publications list
TitleInfluence of PDLA nanoparticles size on drug release and interaction with cells
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsCartaxo A. L., Costa-Pinto A. R., Martins A., Faria S., Gonçalves V. M. F., Tiritan M. E., Ferreira H., and Neves N. M.
Abstract

Polymeric nanoparticles (NPs) are strong candidates for the development of systemic and targeted drug delivery applications. Their size is a determinant property since it defines the NP–cell interactions, drug loading capacity, and release kinetics. Herein, poly(D,L-lactic acid) (PDLA) NPs were produced by the nanoprecipitation method, in which the influence of type and concentration of surfactant as well as PDLA concentration were assessed. The adjustment of these parameters allowed the successful production of NPs with defined medium sizes, ranging from 80 to 460 nm. The surface charge of the different NPs populations was consistently negative. Prednisolone was effectively entrapped and released from NPs with statistically different medium sizes (i.e., 80 or 120 nm). Release profiles indicate that these systems were able to deliver appropriate amounts of drug with potential applicability in the treatment of inflammatory conditions. Both NPs populations were cytocompatible with human endothelial and fibroblastic cells, in the range of concentrations tested (0.187–0.784 mg/mL). However, confocal microscopy revealed that within the range of sizes tested in our experiments, NPs presenting a medium size of 120 nm were able to be internalized in endothelial cells. In summary, this study demonstrates the optimization of the processing conditions to obtain PDLA NPs with narrow size ranges, and with promising performance for the treatment of inflammatory diseases.

Journal J Biomed Mater Res Part A
Volume9999
Pagination1-12
Date Published2018-11-28
PublisherWiley Online Library
ISSN15524965
DOI10.1002/jbm.a.36563
URLhttps://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36563
Keywordscell internalization, Cytocompatibility, PDLA nanoparticles, prednisolone, size distribution
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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