One of the critical challenge facing tissue enginering strategies relate to simultaneous generation of multiple tissues and its functional assembly that lead to complex organ systems. The gradient of structural and mechanical properties present in tissues have led to engineering strategies that focus on this interface. The tissue-to-tissue interface is the most crucial parameter in the approach to multitissue repair. Tissue interfaces present a chemical hierarchical structure leading to different mechanical properties. The interface is the transition between two adjacent tissues as it presents a highly heterogenous structure of chemical and biological organization[2,3]. The hypothesis in this study is that differential functional properities can be engineered by modulation of macromolecules gradient in a cell seeded three-dimensional hydrogel system. Specifically, differential paracrine secretory profiles can be engineered using hBMSC. Hence, the specific objectives of this study are to: assemble the macromolecular gradient from natural materials (gellan gum, hyaluronic acid and collagen); to evaluate whether the gradient is suitable for hMSC encapsulation by cellular viability assessement; and screen the paracrine secretation of hBMSC over time within this gradient hydrogel system.