Biomaterials, Biodegradables and Biomimetics Research Group

Comunication - Oral

Enzymatically crosslinked SF/SF-β-TCP scaffolds incorporating Sr- and Zn-ions as hierarchical structures for osteochondral tissue engineering applications


Introduction. Bilayeredscaffolds consisting of a cartilage-like layer and an underlying bone-like layer have been proposed for osteochondral (OC) tissue applications. Silk fibroin (SF) exhibit high biocompatibility and tunable mechanical propertiesfor OC scaffolding strategies [1].Bioresorbable inorganic materials, such as β-tricalcium phosphate(β-TCP)combined with SF have shown to increase osteogenesis[2]. Herein, the development of novel monolithic and hierarchical scaffoldscombining enzymatically crosslinked SF (HRP-SF) and ZnSr-doped β-TCP, is reported for OC tissue repair/regeneration.


Experimental. The bilayered scaffolds were prepared with 80/20 (w/w) HRP-SF/undoped and ZnSr-doped β-TCP for the bone-like layers, and HRP-SF as cartilage-like layer. Salt-leaching and freeze-drying technologies were applied to induce macro-/micro-porosity to the scaffolds. Physicochemical characterization, structural integrity, and bioactivity of the scaffolds were evaluated. The in vitrocell adhesion, proliferation and extracellular matrix (ECM) production were evaluated by co-culturing human chondrocytes and human osteoblasts in the scaffolds up to 14 days.


Results and Discussion. The results showed an interconnected porosity and homogeneous β-TCP distribution into the subchondral bone layer (Fig.1a and b).The mechanical properties of ZnSr-doped scaffolds were superior than the undoped scaffolds. Co-cultured cells adhered and proliferated on the bilayered scaffolds (Fig. 1c), showing the formation of a mineralized ECM and GAGs deposition in the respective bone and cartilage-like layers.


Conclusions. The structural adaptability and suitable mechanical properties of the proposed engineered OC scaffolds, combined with the biological performance achieved using a co-culturing system, make these scaffolds a viable strategy for OC defects regeneration.



[1] L.-P. Yan, J. Silva-Correia, M. Oliveira, et al., Acta biomaterialia  2015;12:227-241,2015.

[2] S. Pina, R. Canadas, G. Jiménez, et al., Cells Tissues Organs2017;204:150.

Bilayered Scaffold, HRP-mediated silk fibroin hydrogel, Ionicdopants, Osteochondral tissue engineering., β-tricalcium phosphate
Closed Access
Peer Reviewed
Year of Publication
Date Published
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.