Bioactive Macro/micro Porous Silk Fibroin/Nano-sized Calcium Phosphate Scaffolds with potential for Bone Tissue Engineering Applications

last updated: 2013-03-14
TitleBioactive Macro/micro Porous Silk Fibroin/Nano-sized Calcium Phosphate Scaffolds with potential for Bone Tissue Engineering Applications
Publication TypePapers in Scientific Journals
Year of Publication2012
AuthorsYan L. P., Silva-Correia J., Correia C., Caridade S. G., Fernandes E. M., Sousa R. A., Mano J. F., Dr. Oliveira J. M., Oliveira A. L., and Reis R. L.
Abstract

Aim: The development of novel silk/nano-sized calcium phosphate (silk/nano-CaP) scaffolds with highly dispersed CaP nanoparticles in the silk fibroin (SF) matrix for bone tissue engineering. Materials & methods: Nano-CaP was incorporated in a concentrated aqueous SF solution (16 wt.%) by using an in situ synthesis method. The silk/nano-CaP scaffolds were then prepared through a combination of salt-leaching/lyophilization approaches. Results: The CaP particles presented good affinity to SF and their size was inferior to 200 nm when theoretical CaP/silk ratios were between 4 and 16 wt.%, as determined by scanning electron microscopy. The CaP particles displayed a uniform distribution in the scaffolds at both microscopic and macroscopic scales as observed by backscattered scanning electron microscopy and micro-computed tomography, respectively. The prepared scaffolds presented self-mineralization capability and no cytotoxicity confirmed by in vitro bioactivity tests and cell viability assays, respectively. Conclusion: These results indicated that the produced silk/nano-CaP scaffolds could be suitable candidates for bone-tissue-engineering applications.

JournalNanomedicine
Volume8
EditionNanomedicine
Issue3
Pagination359-378
Date Published2012-12-24
PublisherFuture Medicine
ISSN1743-5889
DOI10.2217/nnm.12.118
URLhttp://www.futuremedicine.com/doi/abs/10.2217/nnm.12.118?journalCode=nnm
KeywordsBone Tissue Engineering, Calcium Phosphate, Nanocomposite, Scaffold, Silk Fibroin
RightsopenAccess
Peer reviewedyes
Statuspublished

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