Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Cellular Uptake of Multilayered Capsules Produced with Biomimetic and Genetically Engineered Macromolecules

Abstract

Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape ∼4 μm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72 h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy.

Journal
Acta Biomaterialia
Volume
10
Issue
6
Pagination
2653-2662
Publisher
Elsevier
URL
http://www.sciencedirect.com/science/article/pii/S1742706114000725#
Keywords
Biomaterials, Cellular uptake, layer-by-layer, Multilayer capsules
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Project
N/A
Year of Publication
2014
DOI
10.1016/j.actbio.2014.02.020
Date Published
2014-02-20
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