Chitosan nanofibers as scaffolds for peripheral nerve regeneration

last updated: 2015-11-09
TitleChitosan nanofibers as scaffolds for peripheral nerve regeneration
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2013
AuthorsCarvalho C. R., Silva-Correia J., Martins A., Neves N. M., Oliveira J. M., and Reis R. L.

Damage to the Peripheral Nervous System (PNS) is remarkably com- mon and occurs mainly from trauma or a complication of surgery. Although recovery of nerve function occurs in some mild injuries, out- comes are frequently poor following severe trauma, resulting in long- term impairment of limb function, dysaesthesia and pain, often with associated psychological effects. In contrast to the central nervous sys- tem, the PNS includes an intrinsic capacity to regenerate. Currently, the gold standard autograft repair of the damaged peripheral nerve is far from optimal and is often disappointing. The alternative to the use of grafts is the use of a scaffold that consists in an artificial nerve guide, namely a hollow tube, combined with engineered biomaterials filling its interior in order to provide topographical cues as it has been postu- lated that axon elongation requires guidance by contact with appropri- ate substrates. In the scope of the Biohybrid project, chitosan (CS) powders from Altakitin were supplied and its cytotoxicity was assessed. According to cell viability percentage, no cytotoxicity was observed. Using these biomedical grade powders, biodegradable scaffolds were developed to support neuronal regeneration using the electrospinning technique to produce nanofibers from chitosan solutions. Random and aligned nanofibers were produced and characterized, using techniques such as FTIR, SEM, AFM, DSC and contact angle. To produce a mesh of random chitosan fibers with no beads or other defects, a 5% chitosan solution in trifluoroacetic-acid and dicloromethane as solvents, in the proportion of 70:30 was used. They are super hydrophilic, defect-free and have an average diameter of 184 ` 36 nm. Yarns of aligned chito- san fibers were obtained using the two blades placed in line set up, with the exact same conditions used to obtain random nanofibers. In vi- tro tests to characterize L929 cells viability (MTS assay) and prolifera- tion (DNA quantification) on nanofibers scaffold were performed.

JournalJournal of Tissue Engineering and Regenerative Medicine
Conference NameTERM STEM 2013
Date Published2013-12-01
Conference LocationPorto Palace Congress Hotel&Spa, Porto, Portugal
KeywordsChitosan nanofibers, Peripheral Nerve Regeneration, scaffolds
Peer reviewedno

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