Chondrogenic Differentiation of hASC sub-population in a nude mice model

last updated: 2017-03-07
TitleChondrogenic Differentiation of hASC sub-population in a nude mice model
Publication TypePapers in Scientific Journals
Year of Publication2011
AuthorsRada T., Oliveira J. T., Santos T. C., Castro A. G., Reis R. L., and Gomes M. E.

Cells constitute one of the fundamental components of any cartilage tissue engineering approach. Adipose tis-
sue derived stem cells (ASCs) have a promising future considering the abundance of this tissue in the human body, ease of
harness, and the high number of stem cells that can be isolated from small amounts of tissue. However the stromal vascu-
lar fraction of the adipose tissue that is isolated upon digestion by collagenase followed by a rough selection of the adher-
ent cells, is composed of many different types of cells, some of which may compromise the proliferation and the differen-
tiation of the ASCs. This manuscript reports a study on the in vivo chondrogenic potential of two ASCs specific subpopu-
lations isolated using a method based on immunomagnetic beads coated with specific antibodies. These ASCs subpopula-
tions, isolated using immunomagnetic beads coated with CD29 and CD105 antibodies, were subsequently transfected with
green fluorescent protein (GFP), expanded, and pre-differentiated into the chondrogenic lineage, before being encapsu-
lated in a novel hydrogel based on gellan gum, that has recently been showed to promote in vitro and in vivo cartilage tis-
sue formation. The two ASCs subpopulations encapsulated in the gellan gum hydrogel and in vitro pre-differentiated,
were then subcutaneously implanted in nude mice for 6 weeks. Explants were analyzed by various techniques, namely his-
tology, immunohistology and real time RT-PCR that demonstrated the different behaviour of the two ASCs subpopula-
tions under study, namely their potential to differentiate into the chondrogenic lineage and to form new cartilage tissue.

JournalCurrent Stem Cell Research & Therapy
Date Published2011-07-15
KeywordsAdipose stem cell, cartilage, chondrogenic differentiation
Peer reviewedno

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