Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Compartmentalized Bioencapsulated Liquefied 3D Macro-Construct by Perfusion-Based Layer-by-Layer Technique.

Abstract

Self-supporting, millimeter length 3D constructs consisting of individualized liquefied compartments, were produced using cell encapsulated hydrogel beads as building blocks. A perfusion-based layer-by-layer approach was used that allowed bioencapsulated beads to assemble, pattern, hold and attach to produce non-liquefied 3D constructs with controlled shape, displaying the binding feature of a continuous nanometric multilayer coating. No binders or crosslinking strategies were used. The internal microenvironment of this 3D construct was modified from solid to liquefied state by chelation. MTS and live–dead assays showed enhanced L929 cell viability with liquefied 3D constructs, compared to non-liquefied ones. The proposed technique opens new prospects to create complex 3D polyelectrolyte based constructs for tissue engineering applications.

Journal
RSC Advances
Volume
5
Pagination
2511-2516
Publisher
ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
ISSN
2046-2069
URL
http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C4RA11674G#!divAbstract
Keywords
BEADS, cell microenvironments, ENCAPSULATION TECHNOLOGIES, Hydrogels, Multilayer capsules, SHAPE, TISSUE CONSTRUCTS
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
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