Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

The effect of magnetic stimulation on the osteogenic and chondrogenic differentiation of human stem cells derived from the adipose tissue (hASCs)

Abstract

The use of magnetic nanoparticles (MNPs) towards the musculoskeletal tissues has been the focus of many studies, regarding MNPs ability to promote and direct cellular stimulation and orient tissue responses. This is thought to be mainly achieved by mechano-responsive pathways, which can induce changes in cell behavior, including the processes of proliferation and differentiation, in response to external mechanical stimuli. Thus, the application of MNP-based strategies in tissue engineering may hold potential to propose novel solutions for cell therapy on bone and cartilage strategies to accomplish tissue regeneration.

The present work aims at studying the influence of MNPs on the osteogenic and chondrogenic differentiation of human adipose derived stem cells (hASCs). MNPs were incorporated in hASCs and cultured in medium supplemented for osteogenic and chondrogenic differentiation. Cultures were maintained up to 28 days with/without an external magnetic stimulus provided by a magnetic bioreactor, to determine if the MNPs alone could affect the osteogenic or chondrogenic phenotype of the hASCs.

Results indicate that the incorporation of MNPs does not negatively affect the viability nor the proliferation of hASCs. Furthermore, Alizarin Red staining evidences an enhancement in extracellular (ECM) mineralization under the influence of an external magnetic field. Although not as evident as for osteogenic differentiation, Toluidine blue and Safranin-O stainings also suggest the presence of a cartilage-like ECM with glycosaminoglycans and proteoglycans under the magnetic stimulus provided.

Thus, MNPs incorporated in hASCs under the influence of an external magnetic field have the potential to induce differentiation towards the osteogenic and chondrogenic lineages.

Journal
Journal of Magnetism and Magnetic Materials
Volume
393
Pagination
526–536
Publisher
Elsevier
URL
http://ac.els-cdn.com/S0304885315302109/1-s2.0-S0304885315302109-main.pdf?_tid=57cb53e4-2a20-11e5-9844-00000aab0f27&acdnat=1436875590_8bf49bb0a1a068cb1de2f90acafb4537
Keywords
adipose-derived stem cells, chondrogenic differentiation, Magnetic nanoparticles, osteogenic differentiation, regenerative medicine, Tissue engineering
Rights
Open Access
Peer Reviewed
Yes
Status
published
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