Biomaterials, Biodegradables and Biomimetics Research Group

Conference Abstract -ISI Web of Science Indexed

Evaluation oh the potential of Polyhydroxybutyrate-co-hydroxyvalerate bi-layered scaffolds for skin tissue engineering

Abstract

Polyhydroxybutyrate-co-hydroxyvalerate (PHB-HV) is natural-based polyester produced by microorganisms under unbalanced growth con- ditions. This polymer is known for its biocompatibility and biodegrad- ability and has been explored for tissue engineering approaches. The aim of this work was to create a bi-layered construct, based on PHB- HV, for skin tissue replacement. The PHB-HV bi-layered scaffold was produced taking into consideration the two main skin strata, the epi- dermis and the dermis. For the epidermal fraction, a membrane was developed using a solvent cast approach, while freeze-drying was used to obtain the dermal fraction. The two structures were combined to obtain a scaffold that has a thin compact surface (epidermal) and a por- ous structure (dermis), as confirmed by scanning electron microscopy (SEM) and micro-CT analysis. Each layer of the scaffold, epidermal and dermal, was respectively seeded with human keratinocytes (Kc) and human dermal fibroblasts (Fb). The characterization by SEM con- firmed the typical morphology of Kc and Fb on the expected surfaces. Calcein-AM and DNA quantification showed that both type of cells remained viable along the experiment and were able to attach and pro- liferate on the respective seeding layers. In conclusion, the developed PHB-HV scaffolds have shown suitable properties to sustain the co-cul- ture of Kc and Fb, which constitutes a solid base for further work to demonstrate its potential for skin replacement.

Journal
Journal of Tissue Engineering and Regenerative Medicine
Volume
6
Issue
Sup. 1
Pagination
97-97
Publisher
John Wiley & Sons
URL
http://onlinelibrary.wiley.com/doi/10.1002/term.1586/abstract
Keywords
natural-based polyester, Polyhydroxybutyrate-co-hydroxyvalerate
Rights
Open Access
Peer Reviewed
Yes
Status
published
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.