Inflammatory Response Study of Gellan Gum Impregnated DucFeet Derived Collagen Sponges

last updated: 2016-09-07
TitleInflammatory Response Study of Gellan Gum Impregnated DucFeet Derived Collagen Sponges
Publication TypePapers in Scientific Journals
Year of Publication2016
AuthorsSong J. E., Lee S. E., Cha S. R., Jang N. K., Tripathy N., Reis R. L., and Khang G.

Tissue engineered biomaterials have biodegradable and biocompatible properties. In this study, we have fabricated sponges using duck's feet derived collagen (DC) and gellan gum (GG), and further studied its inflammatory responses. The as-prepared duck's feet DC/GG sponges showed the possibility of application as a tissue engineering material through in vitro and in vivo experiments. The physical and chemical properties of sponges were characterized by compression strength, porosity, and scanning electron microscopy, etc. In vitro cell viability were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. An inflammatory response was studied after seeding RAW264.7 cells on as-fabricated sponges using reverse transcriptase-polymerase chain reaction. In vivo studies were carried out by implanting in subcutaneous nude mouse followed by extraction, histological staining. Collectively, superior results were showed by DC/GG sponges than GG sponge in terms of physical property and cell proliferation and thus can be considered as a potential candidate for future tissue engineering applications.

JournalJournal of Biomaterials Science, Polymer Edition
Date Published2016-08-09
Publisher Taylor & Francis
KeywordsCollagen, Gellan Gum, inflammatory responses, sponge, tissue regeneration
Peer reviewedyes

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