Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform

last updated: 2016-03-30
ProjectNanoTech4ALS :: publications list
TitleInvestigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2016
AuthorsCarvalho M. R., Maia F. R., Reis R. L., and Dr. Oliveira J. M.
Abstract

A microfluidic device such as Kima Pump and Vena8 biochip is able to realize functions that are not easily imaginable in conventional biological analysis, such as highly parallel, sophisticated high-throughput analysis and single-cell analysis in a well-defined manner [1].

Cancer cell tracking within the microfluidic model will be achieved by grafting fluorescent label probe Fluorescein-5(6)-isothiocyanate (FITC) to dendrimer nanoparticles allowing cell visualization by immunofluorescent staining followed by fluorescence microscopy. In this study, synthesis and physicochemical characterization of Carboxymethyl-chitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM NP’s) were performed[2].  Several cancer cell lines such as a HeLA (cervical carcinoma cell line), HTTC-116 (Colon Carcinoma) and Glioblastome cell line (GBM) were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 7d. After finding the adequate NP concentration, the internalization efficiency was tested, as well as cellular trafficking, in static and dynamic conditions (Kima Pump bioreactor).

JournalFrontiers
Conference Name10th World Biomaterials Congress
Date Published2016-03-30
Conference LocationMontreal, Canada
ISSN2296-4185
DOI10.3389
URLhttp://www.frontiersin.org/10.3389/conf.FBIOE.2016.01.02034/event_abstract
KeywordsCancer, Microfluidic, Nanoparticles
RightsopenAccess
Peer reviewedno
Statuspublished

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