Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Liquid Marbles for High-Throughput Biological Screening of Anchorage-Dependent Cells

Abstract

Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fl uorosilanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high-throughput drug screening on anchorage-dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface-modifi ed poly( L -lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self-healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe 3+ is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-te trazolium) colorimetric assay, a well-known method used for the cytotoxicity assessment.

Journal
Advanced Healthcare Materials
Publisher
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
URL
http://onlinelibrary.wiley.com/doi/10.1002/adhm.201400310/abstract
Keywords
High-throughput screening, Hydrophobicity, Image processing, Lliquid marbles, Microbioreactors
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Project
SPECIAL
Year of Publication
2014
DOI
10.1002/adhm.201400310
Date Published
2014-08-04
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