Liquid Marbles for High-Throughput Biological Screening of Anchorage-Dependent Cells

last updated: 2014-09-07
ProjectSPECIAL :: publications list
TitleLiquid Marbles for High-Throughput Biological Screening of Anchorage-Dependent Cells
Publication TypePapers in Scientific Journals
Year of Publication2014
AuthorsOliveira N. M., Correia C. R., Reis R. L., and Mano J. F.

Stable liquid marbles (LM) are produced by coating liquid droplets with a hydrophobic powder. The used hydrophobic powder is produced by fl uorosilanization of diatomaceous earth, used before to produce superhydrophobic structures. Here, the use of LM is proposed for high-throughput drug screening on anchorage-dependent cells. To provide the required cell adhesion sites inside the liquid environment of LM, surface-modifi ed poly( L -lactic acid) microparticles are used. A simple method that takes advantage from LM appealing features is presented, such as the ability to inject liquid on LM without disrupting (self-healing ability), and to monitor color changes inside of LM. After promoting cell adhesion, a cytotoxic screening test is performed as a proof of concept. Fe 3+ is used as a model cytotoxic agent and is injected on LM. After incubation, AlamarBlue reagent is injected and used to assess the presence of viable cells, by monitoring color change from blue to red. Color intensity is measured by image processing and the analysis of pictures takes using an ordinary digital camera. The proposed method is fully validated in counterpoint to an MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-te trazolium) colorimetric assay, a well-known method used for the cytotoxicity assessment.

JournalAdvanced Healthcare Materials
Date Published2014-08-04
PublisherWILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
KeywordsHigh-throughput screening, Hydrophobicity, Image processing, Lliquid marbles, Microbioreactors
Peer reviewedyes

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