Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Modulation of Bone Marrow Mesenchymal Stem Cell Secretome by ECM-Like Hydrogels.

Abstract

Adipose-derived adult stem cells (ASCs), bone marrow mesenchymal stem cells (bmMSCs), and human um- bilical cord perivascular cells (HUCPVCs) tissue have been widely tested for regenerative applications, such as bone regeneration. Moreover, olfactory ensheathing cells (OECs) show promise in promoting spinal cord injury (SCI) regeneration. Our group recently proposed the use of a hybrid scaffold targeting both vertebral bone repair and SCI regeneration. According to this concept, both MSCs and OECs should be in close contact to be influ- enced by the factors that are involved in secretion. For this reason, here we studied the effects of the OEC secretome on the metabolic activity and proliferation of ASCs, bmMSCs, and HUCPVCs. The stem cells' se- cretome effects on metabolic activity and proliferation of the OECs were also considered. In co-cultures of OECs with ASCs, bmMSCs, or HUCPVCs, the metabolic activity/viability, proliferation, and total cell numbers were measured after 2 and 7 days of culture. The results demonstrated that the secretome of OECs has a positive effect on the metabolic activity and proliferation of MSCs from different origins, especially on ASCs. Furthermore, in general, the stem cells' secretome also had a positive effect on the OECs behavior, particularly when ASCs were in co-culture with OECs. These results suggest that the most suitable combination of cells to be used in our hybrid scaffold is the OECs with the ASCs. Finally, this work adds new knowledge to the cell therapy field, bringing new information about paracrine interactions between OECs and distinct mesenchymal stems.

Journal
STEM CELLS AND DEVELOPMENT olume 22, Number 8, 2013
Volume
22
Issue
8
Pagination
1232-1240
Publisher
Mary Ann Liebert, Inc.
URL
http://online.liebertpub.com/doi/abs/10.1089/scd.2012.0524
Keywords
adipose-derived adult stem cells, olfactory ensheathing cells, spinal cord injury
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Year of Publication
2013
DOI
doi: 10.1089/scd.2012.0524
Date Published
2013-04-15
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