Multifunctionalized CMCht/PAMAM Dendrimer Nanoparticles Modulate theCellular Uptake by Astrocytes and Oligodendrocytes in Primary Cultures of Glial Cells

last updated: 2021-01-14
TitleMultifunctionalized CMCht/PAMAM Dendrimer Nanoparticles Modulate theCellular Uptake by Astrocytes and Oligodendrocytes in Primary Cultures of Glial Cells
Publication TypePapers in Scientific Journals
Year of Publication2011
AuthorsCerqueira S. R., Silva B. L., Dr. Oliveira J. M., Mano J. F., Sousa N., Salgado A. J., and Reis R. L.
Abstract

The efficiency of the treatments involving CNS disorders is commonly diminished by the toxicity, reduced stability and lack of targeting of the administered neuroactive compounds. In this study, we have successfully multifunctionalized CMCht/PAMAM dendrimer nanoparticles by coupling the CD11b antibody and loading MP into the nanoparticles. The modification of the new antibody-conjugated nanoparticles was confirmed by S-TEM observation and 1 H NMR and FTIR spectroscopy. Cytotoxicity assays revealed that the conjugates did not affect the viability of both primary cultures of glial and microglial cells. Trace analyses of FITClabelled nanoparticles revealed that the uptake of antibody-conjugated nanoparticles was conserved in microglial cells but significantly decreased in astrocytes and oligodendrocytes. Thus, this study demonstrates that antibody conjugation contributes to a modulation of the internalization of these nanocarriers by different cell types, which might be of relevance for specific targeting of CNS cell populations.

JournalMacromolecular Bioscience
Volume12
Pagination591–597
Date Published2011-08-27
ISSN1616-5195
DOI10.1002/mabi.201100294
Keywordsastrocytes, dendrimer
RightsopenAccess
Peer reviewedno
Statuspublished

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