Optical Projection Tomography as a tool for 3D image tissue engineered products based in hydrogels

last updated: 2014-09-15
TitleOptical Projection Tomography as a tool for 3D image tissue engineered products based in hydrogels
Publication TypePapers in Scientific Journals
Year of Publication2014
AuthorsFigueiras E., Soto A. M., Jesus D., Lehti M., Koivisto J., Parraga J. E., Silva-Correia J., Dr. Oliveira J. M., Reis R. L., Kellomäki M., and Hyttinen J.
Abstract

An Optical Projection Tomography (OPT) system was developed and optimized to image 3D tissue engineered products based in hydrogels. We develop pre-reconstruction algorithms to get the best result from the reconstruction procedure, which include correction of the illumination and determination of sample center of rotation (CoR). Existing methods for CoR determination based on the detection of the maximum variance of reconstructed slices failed, so we develop a new CoR search method based in the detection of the variance sharpest local maximum. We show the capabilities of the system to give quantitative information of different types of hydrogels that may be useful in its characterization.

JournalBiomedical Optics Express
Volume5
Issue10
Pagination3443–3449
Date Published2014-09-05
PublisherOptical Society of America
DOI10.1364/BOE.5.003443
URLhttp://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-10-3443
Keywordsgellan gum-based hydrogels, Optical projection tomography
RightsopenAccess
Peer reviewedyes
Statuspublished

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