The design of bone tissue engineered constructs for future clinical application demands patient customizable 3D structures. These need to be able to sustain the mechanical solicitations, to support cellular functions and to induce bone formation. Our methodology is based on a combination of multiple techniques. This permits us to optimize and customize the final 3D structure at a multi-level scale by using human and marine-origin materials for the production of the primary nano/sub-micro environment that will dictate cell fate. 3D prototyped macro-porous scaffolds where subjected to layer-by-layer assembling and freeze-drying for hierarchical (hier) structuring with nanocoatings and micro-fibrillar structures. Those structures, rich in autologous growth factors (GFs) from human platelet’s lysate (hPL), are stabilized throughout marine-origin polysaccharides functional groups and physical crosslinking. The potential of these nano/microenvironments for the conduction and induction of osteogenic differentiation of human adipose derived stem cells (hASCs) was assessed.