Photocrosslinkable Hyaluronan Hydrogels Incorporating Platelets Lysate for Periodontal Tissue Regeneration Exhibit Mitogenic and Anti-microbial Properties.

last updated: 2016-05-17
ProjectRL3 – TECT :: publications list
TitlePhotocrosslinkable Hyaluronan Hydrogels Incorporating Platelets Lysate for Periodontal Tissue Regeneration Exhibit Mitogenic and Anti-microbial Properties.
Publication TypeConference Abstract -ISI Web of Science Indexed
Year of Publication2015
AuthorsBabo P. S., Pires R. L., Franco A., Santos L., Rodrigues F., Leonor I., Reis R. L., and Gomes M. E.
Abstract

Guided Tissue Regeneration, as well as various combinatory therapies have been employed in the repair of periodontal defects with some success; however an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissues is still to be achieved.

Platelet Lysate (PL) has great potential for tissue regeneration, as source of growth factors involved in essential stages of wound healing and regenerative processes such as chemotaxis, cell proliferation and differentiation1. Herein we report the development an injectable photocrosslinkable hydrogels, prepared from methacrylated Hyaluronic Acid (me-HA) and incorporating PL.

The photocrosslinking reaction, triggered after UV excitation of the photoinitiator Irgacure 2959, produced stable and versatile hydrogels. The viscoelastic properties and resilience to degradation by hyaluronidase of the hydrogels were enhanced with the incorporation of PL. Moreover, human Periodontal Ligament Fibroblasts (hPDLFs), either seeded or encapsulated into the hydrogels, showed enhanced biological performance, proportionally to the amount of PL incorporated. Additionally, the hPDLFs seeded on the surface, tend to migrate into the hydrogels incorporating PL. Remarkably, the PL provided antimicrobial properties against methicillin-resistant Staphylococcus aureus.

The unique mitogenic, chemotatic and prophylaxis-maintenance properties displayed by the photocrosslinkable HA-PL hydrogels here reported, makes them outstanding materials for tissue engineering approaches targeting various tissues, namely the periodontium.

Acknowledgments: Pedro Babo acknowledges FCT for the PhD grant SFRH/BD/73403/2010. This research has received funding from the EC project POLARIS (REGPOT-CT2012-316331), and project RL3 -TECT-NORTE-07-0124-FEDER-000020 cofinanced by ON.2 under NSRF, through the ERDF.

Reference

1. Santo VE, et al. Tissue Engineering B 4, 308, 2013.

JournalTissue Engineering Part A
Conference Name2015 4th TERMIS World Congress
Volume21
IssueS1
PaginationS390
Date Published2015-08-28
ISSN2152-4947
DOI10.1089/ten.tea.2015.5000.abstracts
KeywordsPeriodontal tissue regeneration, Photocrosslinkable hydrogels, Platelet lysate
RightsopenAccess
Peer reviewedyes
Statuspublished

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