Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds

last updated: 2017-03-07
TitlePreliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds
Publication TypePapers in Scientific Journals
Year of Publication2008
AuthorsSalgado A. J., Gomes M. E., Chou A. M., Coutinho O. P., Reis R. L., and Hutmacher D. W.

Up to today, several techniques have been used to produce biodegradable porous scaffolds for tissue engineering. In this work, a new
technique based on extrusion by using blowing agents in combination with a 50:50 (wt.%) blend of starch/cellulose acetate (SCA) was
studied. The results show that by using this technique it was possible to obtain scaffolds with 70% of porosity and a fully interconnected
network of pores, with sizes ranging from 200 to 500 Am. After their production, the mechanical properties of these scaffolds were tested,
presenting a compressive modulus of 124.6F27.2 MPa and a compressive strength of 8.0F0.9 MPa. These values are within the best found
in the literature and show that by using this technique, it is possible to produce scaffolds that, from a mechanical standpoint, may be suitable
for bone tissue engineering. Cell culturing experiments showed that cells were viable and that there were no signs of cellular death after 3
weeks of culture. Finally, biochemical assays demonstrate that cells maintained the osteogenic phenotype throughout the experiment and
deposition of mineralized extracellular matrix could be detected

JournalMaterials Science & Engineering C-Biomimetic and Supramolecular Systems
Date Published2008-11-05
Keywordscell culture, Degradable polymers, Osteoblast, starch-based scaffolds, Tissue engineering
Peer reviewedno

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