Rheological and Mechanical Properties of Acellular and Cellladen Methacrylated Gellan Gum Hydrogels

last updated: 2014-01-09
TitleRheological and Mechanical Properties of Acellular and Cellladen Methacrylated Gellan Gum Hydrogels
Publication TypePapers in Scientific Journals
Year of Publication2013
AuthorsSilva-Correia J., Gloria A., Oliveira M. B., Mano J. F., Dr. Oliveira J. M., Ambrosio L., and Reis R. L.
Abstract

Tissue engineered hydrogels hold great potential as nucleus pulposus substitutes (NP), as they promote intervertebral disc (IVD) regeneration and re-establish its original function. But, the key to their success in future clinical applications greatly depends on its ability to replicate the native 3D micro-environment and circumvent their limitation in terms of mechanical performance. In the present study, we investigated the rheological/mechanical properties of both ionic- (iGG-MA) and photo-crosslinked methacrylated gellan gum (phGG-MA) hydrogels. Steady shear analysis, injectability and confined compression stress-relaxation tests were carried out. The injectability of the reactive solutions employed for the preparation of iGG-MA and phGG-MA hydrogels was firstly studied, then the zero-strain compressive modulus and permeability of the acellular hydrogels were evaluated.In addition, human intervertebral disc (hIVD) cells encapsulated in both iGG-MA and phGG-MA hydrogels were cultured in vitro, and its mechanical properties also investigated under dynamic mechanical analysis at 37ºC and pH 7.4. After 21 d of culturing, hIVD cells were alive (Calcein AM) and the E’ of ionic-crosslinked hydrogels and photo-crosslinked was higher than that observed for acellular hydrogels. Our study suggests that methacrylated gellan gum hydrogels present promising mechanical and biological performance as hIVD cells were producing extracellular matrix.

JournalJournal of Biomedical Materials Research: Part A
Volume101
Issue12
Pagination3438-3446
Date Published2013-04-09
PublisherWiley Periodicals, Inc
URLhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34650/abstract
KeywordsHydrogels, injectability, Mechanical Properties, methacrylated gellan gum, rheology
RightsopenAccess
Peer reviewedyes
Statuspublished

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