Silk Fibroin/Nano-CaP Bilayered scaffolds for Osteochondral Tissue Engineering

last updated: 2013-11-05
TitleSilk Fibroin/Nano-CaP Bilayered scaffolds for Osteochondral Tissue Engineering
Publication TypePapers in Scientific Journals
Year of Publication2014
AuthorsYan L. P., Oliveira J. M., Oliveira A. L., and Reis R. L.

In this study, bilayered silk and silk/nano-CaP scaffolds were developed for osteochondral (OC) tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nano-CaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed that the both layers presented porous structure and integrated well. Micro-computed tomography images confirmed that the CaP phase was only retained in the silk/nano-CaP layer. The hydration degree and mechanical properties of the bilayered scaffold were comparable to the ones of each single layer. The apatite crystal formation was limited to the silk/nano-CaP layer, when soaking the scaffold in a simulated body fluid solution, which is a must for the application of the developed scaffolds in OC tissue engineering.


JournalKey Engineering Materials
Date Published2014-01-30
PublisherTrans Tech Publications, Switzerland
KeywordsBilayered Scaffold, Nano Calcium Phosphate, Osteochondral Tissue Engineering, Silk Fibroin
Peer reviewedyes

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