Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

A two-component pre-seeded dermal-epidermal scaffold

Abstract

We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment.

Journal
Acta Biomaterialia
Volume
10
Issue
12
Pagination
4928-4938
Publisher
Elsevier
ISSN
1742-7061
URL
http://www.sciencedirect.com/science/article/pii/S1742706114003742
Keywords
Amine–aldehyde bonding, Bilayered, Hyaluronic acid, Skin defects
Rights
Open Access
Peer Reviewed
Yes
Status
published
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