In vitro evaluation of the biological performance of macro/ micro-porous silk fibroin and silk-nano calcium phosphate scaffolds

last updated: 2014-09-12
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TitleIn vitro evaluation of the biological performance of macro/ micro-porous silk fibroin and silk-nano calcium phosphate scaffolds
Publication TypePapers in Scientific Journals
Year of Publication2014
AuthorsYan L. P., Dr. Oliveira J. M., Oliveira A. L., and Reis R. L.
Abstract

This study evaluates the biological performance of salt-leached macro/microporous silk scaffolds (S16) and silk- nano calcium phosphate scaffolds (SC16), both deriving from a 16 wt % aqueous SF solution. Enzymatic degradation results showed that the silk-based scaffolds presented desira- ble biostability, and the incorporation of calcium phosphate further improved the scaffolds’ biostability. Human adipose tissue derived stromal cells (hASCs) were cultured onto the scaffolds in vitro. The Alamar blue assay and DNA content revealed that both scaffolds were non-cytotoxic and can support the viability and proliferation of the hASCs. Scanning electron microscopy observation demonstrated that the microporous structure was beneficial for the cell adhesion while the macroporous structure favored the cell migration and proliferation. The histological analysis displayed abun- dant extracellular matrix formed inside the scaffolds, leading to the significant increase of scaffolds’ modulus. These results revealed that S16 and SC16 could be promising alter- natives for cartilage and bone tissue engineering scaffolding applications, respectively. 

JournalJ Biomed Mater Res Part B
Pagination0-0
Date Published2014-08-02
PublisherWiley
DOI10.1002/jbm.b.33267
URLhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33267/abstract
Keywordsnano-calcium phosphates, scaffolds, Silk Fibroin
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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