Biomaterials, Biodegradables and Biomimetics Research Group

Conference Abstract -ISI Web of Science Indexed

Co-assembly of peptide and carbohydrate amphiphiles to generate proteoglycan mimics

Abstract

Peptide amphiphiles (PA) have been used as building blocks that generate nanofibrous protein mimics through self-assembly under physiological conditions. These supramolecular structures are maintained by non-covalent interactions, such as, Pi-Pi stacking, hydrogen bonding and hydrophobic effects. The generated fibers can be further crosslinked via salt bridges thus forming hydrated systems that resemble the extracellular matrix (ECM) at structural and functional level. However, the proteins in the ECM are often presented as glycoconjugates such as glycoproteins and proteoglycans. Carbohydrate-modified PAs are just emerging as alternative or complementary building blocks able to generate closer supramolecular ECM mimics. Such PAs are challenging at synthetic, supramolecular and biofunctional level. Carbohydrates bear different –OH groups prompt to react and thus, different protections are needed for selective functionalization. Moreover, once conjugated to the PA, the carbohydrate moiety can alter its self-assembling capacity, as well as, the biofunctionality of the incorporated bioactive peptide. We therefore developed a simpler approach for generation of minimalistic proteoglycan mimics: co-assembly of short, aromatic PA and their carbohydrate analogues. The nanofibers generated by this approach have a PA core (e.g. fmoc-FF) and a carbohydrate shell (e.g. fmoc-glucosamine-6-phosphate or fmoc-glucosamine-6-sulfate). They present: 1) a higher mechanical performance than the PA single component systems; 2) an improved biofunctionality as demonstrated by our studies with growth factors (e.g. FGF2), lectins and cells.

 

 

Journal
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Volume
256
Pagination
113-CARB
Publisher
ACS
ISSN
0065-7727
URL
https://plan.core-apps.com/acsboston18/abstract/22b86c0c-58bc-4db9-b2a3-18abb28dd0c6
Keywords
glycopeptides, self-assembly, supramolecular gels
Rights
Open Access
Peer Reviewed
Yes
Status
published
Project
CHEM2NATURE
Year of Publication
2018
Date Published
2018-08-19
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