Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Colorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine

Abstract

Awareness that traditional two-dimensional (2D) in vitro and nonrepresentative animal models may not completely
emulate the 3D hierarchical complexity of tissues and organs is on the rise. Therefore, posterior
translation into successful clinical application is compromised. To address this dearth, on-chip biomimetic microenvironments powered by microfluidic technologies are being developed to better capture the complexity of in vivo pathophysiology. Here, we describe a “tumor-on-a-chip” model for assessment of precision nanomedicine delivery on which we validate the efficacy of drug-loaded nanoparticles in a gradient fashion. The model validation was performed by viability studies integrated with live imaging to confirm the dose-response effect of cells exposed to the CMCht/PAMAM nanoparticle gradient. This platform also enables the analysis at the gene expression level, where a down-regulation of all the studied genes (MMP-1, Caspase-3, and Ki-67) was observed.
This tumor-on-chip model represents an important development in the use of precision nanomedicine toward
personalized treatment.

Journal
Science Advances
Volume
5
Publisher
American Association for the Advancement of Science.
ISSN
2375-2548
URL
https://advances.sciencemag.org/content/5/5/eaaw1317.full
Keywords
3D, Cancer, Microfluidics, Nanoparticles
Rights
Open Access
Peer Reviewed
Yes
Status
published
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