Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

last updated: 2018-05-25
ProjectBioHybrid :: publications list
TitleGellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsCarvalho C. R., Wrobel S., Meyer C., Branderberger C., Cengiz I. F., López-Cebral R., Silva-Correia J., Ronchi J., Reis R. L., Grothe C., Dr. Oliveira J. M., and Haastert-Talini K.
Abstract

Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner. Four different GG formulations were produced by combining varying amounts of High-Acyl GG (HA-GG) and Methacrylated GG (MA-GG). The effective porosity of the freeze-dried networks was analysed by SEM and micro-CT 3D reconstructions, while the degradation and swelling abilities were characterized in vitro for up to 30 days. The metabolic activity and viability of immortalized Schwann cells seeded onto the freeze-dried networks were also evaluated. Finally, the developed hydrogel formulations were freezedried within the chitosan nerve guides and implanted in a 10 mm rat sciatic nerve defect. Functional and histomorphological analyses after 3, 6, and 12 weeks in vivo revealed that although it did not result in improved nerve regeneration, the NGC25:75 formulations could provide a basis for further development of GG scaffolds as luminal fillers for hollow nerve guidance channels.

JournalBIOMATERIALS SCIENCE
Volume6
Issue6
Pagination1059-1075
Date Published2018-05-01
PublisherThe Royal Society of Chemistry
ISSN45343
DOI10.1039/c7bm01101f
URLhttp://pubs.rsc.org/en/content/articlehtml/2018/BM/C7BM01101F
KeywordsBiomaterials, Gellan Gum, Peripheral Nerve Regeneration
RightsopenAccess
Peer reviewedyes
Statuspublished

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