GG-based material with TGF-β to modulate fibroblast-to-myofibroblast transition in scarring control

last updated: 2019-12-17
ProjectECM_INK :: publications list
TitleGG-based material with TGF-β to modulate fibroblast-to-myofibroblast transition in scarring control
Publication TypeComunications - Poster
Year of Publication2019
AuthorsMandon C. A., Pires J., da Silva L. P., Reis R. L., and Marques A. P.

In wound healing in adults, scar formation represents a failure that occurs during the restoration leading to tissue repair rather than regeneration. Understanding the mechanisms underlying scaring is increasingly important to develop specific therapeutics. A way to study this process is to focus on the fibroblast-to-myofibroblast transition, since they seem to be the player in the disturbed adult skin healing. Considering this, the present study proposes an extracellular matrix (ECM)-like material linked to TGF-β1 and TGF-β3 as better 3D system to understand the influence of these growth factors in the fibroblast-to-myofibroblast transition. Gellan gum (GG) was covalently or non-covalently modified following several approaches with the purpose of developing a GG-based hydrogel capable of binding TGF-β1 and TGF-β3, implicated in the fibrotic scarring response and the scarless healing process, respectively. Non-covalent binding resulted from the electrostatic interaction of the growth factors with the GG. GG was chemically modified with sodium periodate and 1-ethyl-3-(3-dimethylaminopropyl) carbodimide (EDC), prior the TGF-β1 and TGF-β3 grafting. Modified-gellan gum-TGF-β1 formulations were analysed by Proton-Nuclear Magnetic Resonance (1H-NMR) and by Fourier Transformed Infrared (FTIR). The amount of growth factor linked to GG was quantified by ELISA. The results confirmed a successful chemical binding of TGF-β1 and TGF-β3 to GG. Non-covalent binding of growth factors to GG was also detected by ELISA although at a lower scale. Overall, the obtained results showed that the properties of the modified GG-based hydrogels using different approaches has potential to work as suitable TGF-β1 and TGF-β3 containing system. This system could be of use to further investigate the implication of these growth factors in the scar formation by modulating their ratio.

Conference NameTERM STEM 2019
Date Published2019-11-06
Conference LocationBraga, Portugal
Keywordsfibroblast-to-myofibroblast transtition, Gellan Gum, scarring, TGF-β
Peer reviewedno

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