Injectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration

last updated: 2019-01-25
TitleInjectable hydrogel composite containing modified gold nanoparticles: implication in bone tissue regeneration
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsLee D., Heo D. N., Nah H. R., Lee S. J., Ko W. K., Lee J. S., Moon H. J., Bang J. B., Hwang Y. S., Reis R. L., and Kwon I. K.

Background: For effective bone regeneration, it is necessary to implant a biocompatible scaffold that is capable of inducing cell growth and continuous osteogenic stimulation at the defected site. Here, we suggest an injectable hydrogel system using enzymatic cross-linkable gelatin (Gel) and functionalized gold nanoparticles (GNPs).
Methods: In this work, tyramine (Ty) was synthesized on the gelatin backbone (Gel-Ty) to enable a phenol crosslinking reaction with horseradish peroxidase (HRP). N-acetyl cysteine (NAC) was attached to the GNPs surface (G-NAC) for promoting osteo-differentiation. 
Results: The Gel-Ty hydrogels containing G-NAC (Gel-Ty/G-NAC) had suitable mechanical strength and biocompatibility to embed and support the growth of human adipose derived stem cells (hASCs) during a proliferation test for three days. In addition, G-NAC promoted osteo-differentiation both when it was included in Gel-Ty and when it was used directly in hASCs. The osteogenic effects were demonstrated by the alkaline phosphatase (ALP) activity test.
Conclusion: These findings indicate that the phenol crosslinking reaction is suitable for injectable hydrogels for tissue regeneration and G-NAC stimulate bone regeneration. Based on our results, we suggest that Gel-Ty/G-NAC hydrogels can serve both as a biodegradable graft material for bone defect treatment and as a good template for tissue engineering applications such as drug delivery, cell delivery, and various tissue regeneration uses.

JournalInternational Journal of Nanomedicine
Date Published2018-11-01
KeywordsEnzymatic cross-linking, Gelatin, N-acetyl cysteine, nanomaterial, osteogenesis
Peer reviewedyes

Back to top