Manganese dioxide-based nano/microreactors for long-term MRI imaging and Reactive Oxygen Species scavenging

last updated: 2020-01-14
ProjectNanoTech4ALS :: publications list
TitleManganese dioxide-based nano/microreactors for long-term MRI imaging and Reactive Oxygen Species scavenging
Publication TypeComunications - Poster
Year of Publication2019
AuthorsLopes S. V., Oliveira E. P., Reis R. L., Silva-Correia J., and Oliveira J. M.

In a healthy brain, manganese (Mn2+) ions can enter excitable cells and circulate within axons and across synapses. Because these ions can also be used as a contrast agent, Manganese Enhanced Magnetic Imaging (MEMRI) has been used, not only to study Amyotrophic Lateral Sclerosis (ALS), but also to guide intrathecal drug/cell delivery [1]. Mn2+ ions allow long-term T1-weigthed imaging, representing an advantage when compared to other ions. In ALS, motor neuron degeneration occurs and it has been correlated with oxidative stress, which can potentially be a therapeutic target [1]. Previous work [2] has demonstrated the ability of manganese dioxide (MnO2) nanoparticles to scavenge Reactive Oxygen Species (ROS) by breaking down hydrogen peroxide in water, oxygen and Mn2+. As MnO2 nanoparticles present toxicity when administered in high doses, it is our goal to develop MnO2 nano/microreactors, where MnO2 nanoparticles will be blended with a methacrylated gellan-gum (GG-MA) matrix. These nano/microreactors will potentially be used, not only to regulate apoptosis and inflammation in tissues, but also to be delivered and guided by MRI. GG-MA was selected due to its affinity to Mn2+, biocompatibility, biodegradability, easy functionalization and similarity to the extracellular matrix [3]. GG-MA was synthesized according to a previously described protocol [4] and further characterized via FTIR and 1H-NMR. A comparison between synthetized and commercially bought MnO2 nanoparticles will also be performed. Nano/microreactors will be evaluated regarding their rheology, stability, permeability and morphology. Their effect on cell viability and ability to scavenge ROS will also be evaluated.

Conference NameTERMSTEM 2019 Conference
Date Published2019-11-06
Conference LocationBraga, Portugal
KeywordsGG-MA, MnO2 nano/microreactors, MRI imaging, ROS scavenging
Peer reviewedno

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