Microparticles and Hydrogels for Ischemic Stroke Imaging and Recovery

last updated: 2020-01-14
ProjectNanoTech4ALS :: publications list
TitleMicroparticles and Hydrogels for Ischemic Stroke Imaging and Recovery
Publication TypeComunications - Poster
Year of Publication2019
AuthorsOliveira E. P., Silva-Correia J., Kalkowski L., Malysz-Cymborska I., Golubczyk D., Kwiatkowska A., Walczak P., Reis R. L., and Oliveira J. M.

Stroke patients suffer impairments for life, as current treatments are mostly palliative and offer unsatisfactory results regarding brain regeneration1,2. The present work aims to develop an approach for Mesenchymal Stem Cells (MSCs) transplantation that makes use of injectable hydrogels, namely Mn2+-crosslinked Thiolated Hyaluronic Acid and methacrylated Gellan Gum. The cell-laden hydrogels will aid neural repair, while Mn2+ incorporation will allow their MRI imaging and investigate their fate in vivo. Growth-factor loaded Nanoparticles will be added to the hydrogels to support host/transplanted cells. These scaffolds will be tested in an animal model of stroke, obtained by microparticles (MPs) containing Mn2+/iron-oxide, which will be injected to produce an artificial brain clog for 30 minutes, originating a stroke-like injury. The MPs were injected through catheters of two sizes without clogging and maintained their shape. The MRI analysis showed that Mn2+/Iron-oxide are good candidates for MRI contrasts, as from 0.5 to 2 mg/mL they produced a signal in T1/T2. Future work will consist on optimizing its release rate, the analysis of hydrogel’s mechanical properties and their biocompatibility with MSCs.

Conference Name1st Discoveries Forum on Regenerative and Precision Medicine
Date Published2019-09-25
Conference LocationPorto
KeywordsBiomaterials, MRI, Stem cells, Stroke, Tissue engineering
Peer reviewedno

Back to top