Tissue engineering approaches for musculoskeletal regeneration that rely on the use of personalized implants are still pursuing the “perfect” biomimetic scaffold [1]. In these strategies, by using promising biomaterials-based inks combined with 3D printing, it is possible to control the architecture of the produced scaffolds and thus, reproduce tissue’s anatomy with a high degree of precision, resolution and reproducibility [2]. In this work, a novel fast-setting bioink based on enzymatically crosslinked silk fibroin (SF) [3] was developed for 3D printing of patient-specific implants.