Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Optimization of high purity chitin and chitosan production from Illex argentinus pens by a combination of enzymatic and chemical processes

Abstract

The present report illustrates the optimisation of the experimental conditions for the chemical and enzymatic production of chitin and chitosan from Illex argentinus pen by-products. Optima conditions for chitin isolation were established at 0.82 M NaOH/36.4 °C, 57.5 °C/pH = 9.29, 59.6 °C/pH = 9.30 and 49.6 °C/pH = 5.91 for chemical, alcalase, esperase and neutrase deproteinization, respectively. Chitin samples were subsequently deacetylated by alkaline treatment reaching the highest degrees of deacetylation (DD > 93%) at 61.0–63.7% of NaOH and 14.9–16.4 h of hydrolysis depending on the type of process previously performed to the squid pens. Molecular weight (as number average molecular weight, Mn) of chitosan produced in the experimental designs ranged from 143 kDa (PDI 2.37) to 339 kDa (PDI 2.38).

Journal
Carbohydrate Polymers
Volume
174
Pagination
262-272
Publisher
Elsevier
ISSN
0144-8617
URL
http://www.sciencedirect.com/science/article/pii/S0144861717307087
Keywords
chitin, Chitosan, Illex argentinus
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
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