Osteogenesis evaluation of duck’s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone

last updated: 2017-02-27
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TitleOsteogenesis evaluation of duck’s feet derived collagen/hydroxyapatite sponges immersed in dexamethasone
Publication TypePapers in Scientific Journals
Year of Publication2017
AuthorsKook Y. J., Lee D. H., Song J. E., Tripathy N., Jeon Y. S., Dr. Oliveira J. M., Reis R. L., and Khang G.
Abstract

Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone.

Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex()DC and Dex()-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex()DC, Dex()-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR).

Results: In this study, we assessed osteogenic differentiation of BMSCs on Ducks feet-derived collagen (DC)/ HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days.

Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.

JournalBiomaterials Research
Volume21
Issue2
Pagination1-7
Date Published2017-01-02
PublisherBioMed Central
ISSN2055-7124
DOI10.1186/s40824-017-0088-4
Keywordsbone, Collagen, hydroxyapatite
RightsopenAccess
Peer reviewedyes
Statuspublished

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