Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Retinoic Acid Benefits Glomerular Organotypic Differentiation from Adult Renal Progenitor Cells In Vitro

Abstract

When in certain culture conditions, organotypic cultures are able to mimic developmental stages of an organ, generating higher- order structures containing functional subunits and progenitor niches. Despite the major advances in the area, researchers have not been able to fully recapitulate the complexity of kidney tissue. Pluripotent stem cells are extensively used in the field, but very few studies make use of adult stem cells. Herein, we describe a simple and feasible method for achieving glomerular epithelial differentiation on an organotypic model comprising human renal progenitor cells from adult kidney (hRPCs). Their glomerular differentiative potential was studied using retinoic acid (RA), a fundamental molecule for intermediate mesoderm induction on early embryogenesis. Immunofluorescence, specific cell surface markers expression and gene expression analysis confirm the glomerular differentiative potential of RA in a short-term culture. We also compared the potential of RA with a potent WNT agonist, CHIR99021, on the differentiative capacity of hRPCs. Gene expression and immunofluorescence analysis confirmed that hRPCs are more sensitive to RA stimulation when compared to CHIR9901. Endothelial cells were also included on the spheroids, resulting in a higher organizational level. The assembly potential of these cells and their selective stimulation will give new insights on adult organotypic cell culture studies and will hopefully guide more works in this important area of research.

Journal
Stem Cell Reviews And Reports
Volume
-
Edition
-
Issue
-
Pagination
-
Publisher
Springer
ISSN
2629-3269
URL
https://link.springer.com/article/10.1007/s12015-021-10128-8
Keywords
Adult progenitor cells, organotypic models, Renal differentiation, Retinoic acid, spheroids, WNT
Rights
Closed Access
Peer Reviewed
Yes
Status
published
Project
Cells4_IDs
Year of Publication
2021
DOI
10.1007/s12015-021-10128-8
Date Published
2021-01-21
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