Silk fibroin/amniotic membrane 3D bi-layered artificial skin

last updated: 2018-09-24
ProjectFoReCaST :: publications list
TitleSilk fibroin/amniotic membrane 3D bi-layered artificial skin
Publication TypePapers in Scientific Journals
Year of Publication2018
AuthorsGholipourmalekabadi M., Samadikuchaksaraei A., Seifalian A. M., Urbanska A. M., Ghanbarian H., Hardy J. G., Omrani M. D., Mozafari M., Reis R. L., and Kundu S. C.

Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for skin regeneration.

JournalBiomedical Materials
Date Published2018-02-20
Keywordshuman amniotic membrane, regenerative medicine, Scaffold, Silk Fibroin, skin
Peer reviewedyes

Back to top